Photocaged probes for spatiotemporal imaging
WebJul 8, 2024 · The U.S. Department of Energy's Office of Scientific and Technical Information WebMay 12, 2024 · Photocaged DNAzymes can be activated by light for sensing purposes that need spatial and temporal resolution. Studying complex biological processes requires logic photocontrol, but unfortunately all the literature-reported photocaged DNAzymes working in live cells cannot be selectively controlled by light irradiation at different wavelengths.
Photocaged probes for spatiotemporal imaging
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WebEarly cancer diagnosis is of great significance to relative cancer prevention and clinical therapy, and it is crucial to efficiently recognize cancerous tumor sites at the molecular … Webvalidate the photorelease of photocaged TAM,22 it required near toxic concentrations (Z8 mM). Consequently, our initial test of ONB–4OHT compounds used similarly high concentrations (5 mM), and we often observed reduced cell viability after treatment (Fig. 1d). Therefore, we sought to re-optimize condi-tions for use with photocaged 4OHT.
WebHere, we report a strategy to prevent premature binding through the development of photo-conditional intrabodies. Using genetic code expansion, we introduce photocaged amino acids within the nanobody-binding interface, which, after photo-activation, show instantaneous binding of target proteins with high spatiotemporal precision inside living ... WebDec 15, 2024 · This approach enables protein glycation to be controlled with spatiotemporal precision within live cells and expands the chemical toolbox needed to elucidate the roles of glycated proteins across various pathologies. ... Photocaged dicarbonyl probe provides spatiotemporal control over protein glycation A. K. Hurben, P. Ge, ...
WebJan 4, 2024 · Recent advances in fluorescence imaging techniques and super-resolution microscopy have extended the applications of fluorescent probes in studying various cellular processes at the molecular level. Specifically, organelle-targeted probes have been commonly used to detect cellular metabolites and transient chemical messengers with … WebDec 22, 2024 · Present here is a novel photocaged amplified FRET nanoflare (PAFN), which spatiotemporal controls of mRNA-powered nanomachine for precise and sensitive miRNA …
WebPhotocaged probes for spatiotemporal imaging. Sensors and Actuators B: Chemical 2024, 288, 113-119. DOI: 10.1016/j.snb.2024.02.078. Min Liu, Yaqian Liu, Fan Wu, Yuhao Du, Xiang Zhou. Specific stabilization of DNA G-quadruplex structures with a chemically modified complementary probe. Bioorganic & Medicinal ...
WebDiketopyrrolopyrrole-based fluorescence probes for the imaging of lysosomal Zn 2+ and identification of prostate cancer in human tissue. Chemical Science 2024, 10 (22 ... Photocaged probes for spatiotemporal imaging. Sensors and Actuators B: … rockwell software hardware maintenance toolWebDec 15, 2024 · Protein glycation is a disease associated, non-enzymatic, posttranslational modification generated by endogenous dicarbonyl metabolites. Currently, there is a lack … rockwell software rsviewWebJun 7, 2024 · To improve detection accuracy and achieve on-demand sensing, herein, we developed photocaged FRET nanoflares for spatiotemporal microRNA imaging in living cells. In other words, the probes will not work until they are exposed to UV light. otterbox thin flex z fold 3WebOct 12, 2016 · Photoactivatable fluorophores have become an important technique for the high spatiotemporal resolution of biological imaging. Here, we developed a novel … otterbox thin flex series caseWebBoth probes can be efficiently uncaged via irradiation to produce the active C-nucleophile probe DYn-2. Labeling assay in living cells demonstrated DYn-2-ONB exhibited better … otterbox to fit samsung galaxy grand primeWebIn 2015, we proposed FRET nanoflares for intracellular molecular (RNA, H+, and K+) detection. To improve detection accuracy and achieve on-demand sensing, herein, we developed photocaged FRET nanoflares for spatiotemporal microRNA imaging in living cells. In other words, the probes will not work until they are exposed to UV light. 展开 otterbox thin flex z flip 4WebJun 16, 2024 · Remote activation of biomarker sensing holds a great promise of shifting the success of in vitro diagnostics to spatiotemporally controlled in vivo visualization of tumor, and in turn, imaging guided therapy. Herein, a “dual-key-one-lock” nanodevice was designed and built by assembling thermo-activatable probe of trimeric DNA hybrids into a … rockwell software download windows 10