Phosphorescence lifetime imaging

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August undefined, 2024

WebJun 12, 2015 · Phosphorescence lifetime imaging of OC inside a single cell. Figure 1 compares OC imaging in MKN45 cells using the previous wide-field fluorescence microscope 14 and a confocal microscope (i.e ... WebAug 13, 2024 · The reversible tracking of introduced exogenous labile Zn2+ in MCF-7 and HeLa cells via phosphorescence imaging and phosphorescence lifetime imaging (PLIM) …

Two-Photon Phosphorescence Lifetime Microscopy SpringerLink

WebMotivation of Using Phosphorescence Lifetime Imaging. Phosphorescence occurs when an excited molecule transits from the first excited singlet state, S1, into the first triplet state, T1, and returns from there to the ground state by emitting a photon [24]. Both the S1-T1 transition and the T1-S0 transition are forbidden processes. WebFeb 3, 2024 · Phosphorescence lifetime imaging microscopy (PLIM) measurements of HeLa cells co-stained with PPYDM and hydrophilic BTP-PEG 48 allowed for the evaluation of intracellular and extracellular O 2 levels in cell culture. dake\u0027s annotated reference bible

Two-Photon Phosphorescence Lifetime Microscopy - PubMed

WebContrasting these methods, Fluorescence Lifetime Imaging Microscopy (FLIM) delivers information about changes in the binding, conformation, and composition of biological samples that are not accessible using other techniques. It stands out as the most rigorous method for measuring: Molecular environment parameters; WebDec 5, 2024 · Taking advantage of the large Stokes shift and long lifetime of phosphorescence, there are three types of phosphorescence imaging methods: (1) Different-channel phosphorescent imaging. As the phosphorescence has a Stokes shift. Compared with the fluorescence, it can be collected by different filters bands for … WebApr 10, 2024 · Accordingly, we categorized the lowest excited triplet state as short phosphorescence (SPhos.) and long phosphorescence (LPhos.), respectively, according to whether the phosphorescence lifetime is ... biotene candy

Research progress of phosphorescent probe for biological imaging

Fluorescence and phosphorescence lifetime imaging …

WebDec 4, 2024 · The fluorescence lifetime of a fluorophore depends on its molecular environment but not concentration, and fluorescence lifetime imaging (FLIM) can reflect the alterations in microenvironments with high accuracy. 7 In the FLIM technique, molecular rotors with viscosity-dependent lifetimes can map cellular viscosity quantitatively. 8,9 … WebJan 20, 2024 · Furthermore, the phosphorescence lifetime data indicated a bi-exponential signal decay with both a faster few µs decay time in the range of 3–10 µs and a slower one in the range of 30–60 µs.... dake\u0027s study bible free appWebDec 3, 2024 · Phosphorescence lifetime imaging microscopy (PLIM) combined with an oxygen (O 2)-sensitive luminescent probe allows for high-resolution O 2 imaging of living tissues. Herein, we present phosphorescent Ir(III) complexes, (btp) 2 Ir(acac-DM) (Ir-1) and (btp-OH) 3 Ir (Ir-2), as useful O 2 probes for PLIM measurement. These small-molecule … biotene antibacterial mouthwash

"WebNov 19, 2024 · For the first time, the time-resolved two-photon excited autophosphorescence of non-labeled biological specimens was investigated by phosphoresce lifetime imaging with microscopic spatial resolution. A modified multiphoton tomograph was employed to record both photoluminescence contributions, … " - Phosphorescence lifetime imaging

Phosphorescence lifetime imaging

High resolution imaging of intracellular oxygen concentration by ...

http://sioc-journal.cn/Jwk_hxxb/EN/10.6023/A23030077

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WebOrganic room-temperature phosphorescence (RTP) materials are currently the focus of research in the field of bioimaging. In comparison with the conventional imaging modalities based on organic fluorescent dyes, RTP materials with long lifetime enable time-resolved imaging to improve the imaging resolution by avoiding autofluorescence. A Nikon Ti-E microscope equipped with a Becker & Hickl 405 nm 40 ps diode laser (variable repetition rate 1–80 MHz). PLIM Images were acquired with a 60X (NA 1.20) water immersion objective. PLIM data were acquired using a Becker & Hickl DCS120 confocal unit and a TCSPC SPC150 (Becker & Hickl GmBH) … See more All reagents, unless otherwise stated, were purchased from Thermofisher Scientific. A stock solution of GFP (a kind gift from Prof Cameron Nolan, Science and … See more Recombinant DNAs (cDNA) of Ku70 and Ku80 were bought from Origene, Maryland, USA. The linearized vectors, pOPINE (from Oxford Protein Production Facility, … See more All cell culture reagents, unless otherwise stated, were purchased from Thermofisher Scientific. Cells were grown under humidified 5% CO2in air at 37 °C and … See more Ku80-GFP stably expressing V15B cells (a gift from Dr. Dick van Gent) were passaged grown in phenol red free minimal essential medium (MEM) supplemented with … See more

WebAug 10, 2024 · The long-lifetime phosphorescence is easily discriminated from short-lived background noises of autofluorescence and scattered lights [ 12 ]. Time-gated acquisition of the phosphorescence signals thus enables complete elimination of the noise in the images, producing high spatial resolution. WebApr 7, 2024 · A set of phosphorescent materials were evaluated using a new macro-imager based on fast time-stamping Tpx3Cam camera with phosphorescent lifetime imaging (PLIM) capabilities.

WebMay 7, 2024 · In this work, a dual-emissive phosphorescent polymer temperature probe, composed of iridium(III) complexes as temperature sensitive unit with phosphorescence … WebAbstract. Probes featuring room-temperature phosphorescence (RTP) are promising tools for time-resolved imaging. It is worth noting that the time scale of time-resolved …

WebOct 28, 2024 · As long as the burst duration is shorter than the phosphorescence lifetime the excitation efficiency will increase in proportion to the number of pulses within the burst. Multi-pulse excitation has been used for multiphoton phosphorescence imaging earlier but bh were first to apply it to TCSPC PLIM. The principle is shown in Fig. 2.2. The ...

WebOct 28, 2024 · The phosphorescence lifetime image is obtained by building up a similar distribution over the times, T, within the laser modulation period and the beam position, x, … biotene chewing gum recallWebMay 30, 2024 · Abstract. Two-photon Phosphorescence Lifetime Microscopy (2PLM) is an emerging nonlinear optical technique that has great potential to improve our … biotene alcohol free mouthwashWebSep 1, 2024 · Simultaneously, the O 2 -sensitive phosphorescent lifetimes of Re-dca 2 can be utilized for PLIM imaging of intracellular oxygen consumption, thus reflecting the Re-dca 2 induced glycolysis-to-glucose oxidation reversal at the early drug treatment stage. biotene bottle sizeWebDec 30, 2024 · In the field of fluorescence microscopy, there is continued demand for dynamic technologies that can exploit the complete information from every pixel of an … biotene bad breathWebTwo-photon Phosphorescence Lifetime Microscopy (2PLM) is an emerging nonlinear optical technique that has great potential to improve our understanding of the basic biology underlying human health and disease. Although analogous to 2-photon Fluorescence Lifetime Imaging Microscopy (2P-FLIM), the cont … biotene antibacterialWebDec 3, 2024 · Phosphorescence lifetime imaging microscopy (PLIM) combined with an oxygen (O 2 )-sensitive luminescent probe allows for high-resolution O 2 imaging of living … dake\u0027s annotated reference bible onlineWebApr 23, 2024 · Recently, two-photon phosphorescence lifetime microscopy (2PLM) technique has been used to quantitatively measure oxygen pressure in the cerebral vasculature and in the bone marrow, [ 9] but the method based on multiphoton scanning microscope needs ultrafast laser with high excitation power and can only perform … biotene and thyroid